ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING BACTERIA FROM DIFFERENT RHIZOSPHERIC SOILS

The production of indole acetic acid (IAA) is a significant property rhizospheric bacteria to stimulate and promote plant growth. In this investigation, acid-producing from the rhizosphere five distinct plants (Wheat, Gulchen, Rose, Lady Finger, Bouginvella) were isolated characterized. Bacterial strains identified by morphological characterization. Out 30 bacterial isolates, 5 isolates selected as efficient producers Indole acid. These subjected several biochemical tests for identification at genus level. After determination genus, antibiotic sensitivity test was performed check resistance pattern each isolate against antibiotics. Selected characterized using molecular techniques. DNA extracted WizPrepTMg mini kit amplification 16S rRNA gene done PCR. Amplification PCR product confirmed agarose gel electrophoresis. From tests, Klebsiella spp., E. coli, Bacillus sp. tested positive. spp. showed 87, 88, 81, 91, 100, 90, 87% enrofloxacin, gentamycin, penicillin, ampicillin, chlotetracyclin, tylosine, oxytetracycline, amoxicillin, respectively. coli 80, 79, 84, 86, 82, 89% amoxicillin respectively whereas 93, 89 100% shown All these found positive pneumonia RY6, oxytoca strain RY8, RY10, Escherichia RY20, tequilensis RY23. It concluded that synthesizing IAA considered an effective tool isolation characterization have role in promoting